Strategies for the Multiplication of Male Sterile Lines

Although the described systems have provided important information about anther and pollen development, and ways to interfere with it, their potential use for commercial hybrid seed production is often limited because of the lack of

ß-ketothiolase acetyl-CoA

acetoacetyl-CoA

Normal anther development male fertile plant

Distorted anthers male sterile plant

Fig. 14.3 Engineering male sterility with b-ketothiolase. (a) In chloroplasts, acetyl-CoA is normally converted by acetyl-CoA carboxylase to yield malonyl-CoA. (b) In transgenic plants expressing high amounts of b-ketothiolase, this enzyme out-competes acetyl-CoA carboxylase converting acetyl-CoA into acetoactyl-CoA. As a consequence, anther development is impaired. Based on data from Ruiz and Daniell (2005)

cost-effective and efficient methods to multiply the engineered male-sterile plants (for an overview of multiplication strategies, see Perez-Prat and van Lookeren Campagne 2002).

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