The production of transgenic plants via transformation has tremendously impacted agriculture and the face of our planet. Through improvements in technology for DNA introduction, production of transgenics is no longer as limiting as it once was. Determination of potential GOIs as well as evaluation of transgenics is becoming a new bottleneck in plant biotechnology. However, improvements in DNA introduction methodology as well as developing predictable transgene expression models are still needed. Transformation efforts should not be curtailed until all plants of interest can be transformed with the same ease as Arabidopsis.

Acknowledgements Salaries and research support were provided by the United Soybean Board and by State and Federal funds appropriated to The Ohio State University/Ohio Agricultural Research and Development Center (OSU/OARDC). Mention of proprietary products does not constitute a guarantee or warranty of the product by OSU/OARDC and also does not imply approval to the exclusion of other products that may also be suitable.

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