These are performed according to internationally accepted rules established by the International Seed Testing Association (ISTA) using prescribed temperature regimes. Example regimes could be either continuous 20°C or alternating 20/30°C during dark and illuminated periods. Lighting regimes are also standardized. Radicle emergence is recorded daily for the first 5 days of incubation, and counts of normal and abnormal seedlings are made after 10 days by suitably qualified seed analysts.
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