Procedure for Exercise C
- Select three different herbs for your herb garden.
- Obtain six^pots and six labels, two for each herb.
Chi î. Division and Cloning 19
- You will l>c preparing two cuttings from each herb. One cutting ofcach will be dipped in a rooting hormone, while the second will be the untreated control. Put vour initials and the name of the herb on each label. On the label also put a ( + ) for the cut ting dipped in the hormone and a (-) for the untreated control.
- Fill the pots with the potting soil, and add water. Use a pencil to make a hole in the soil.
- Cuttings should be made with a fresh razor blade at a 45° angle to the stem.
- Make cuttings from cach herb you have selected. Each cutting should be the top S to 10 cm of a different branch.
- Remove any flowers or flower buds from die cutting. This will help the cutting use all the stored carbohydrates for root formation.
- Remove the bottom two leaves from cach cutting.
- Dip one cutting of each type in Rootonc. This is a hormone that promotes rooting in plants that arc difficult to root. Gently tap the cutting to remove any excess Rootonc.
- Insert the cuttings in the holes you have made in the soil. Each cutting should be inserted into the potting mix to a depth one-third to one-half its length. Make sure the cutting is vertical and upright. Gently pack the soil around the stem. Place the appropriate label in each pot.
- Water again, and then place the pots on a tray.
- Piacc rhc trays in a well-lighted area, but avoid direct sun. Keep the soil moist until the cuttings have rooted (they may need to be watered two or three times each week). Your instructor may want you to cover the trays with a clcar plastic sheet to minimize water loss.
- After 2 weeks, gently dig up cach cutting and very carefully rinse off any clinging soil. In table 1 of worksheet 2 2, record whether roots arc present or not for each cutting.
- We will also combine the results from the whole class. Express the class results as a percent of cuttings that developed roots for the treated and control of each herb. Enter the class results in table 2 on worksheet 2-2.
- Quickly repot the cuttings in soil, and continue watering at least once a week. Wotr. Depending on the growing conditions in your laboratory, it may be necessary to water more frequently. Your instructor will provide guidance on this.) These plants can be used again for Laboratory Topic 17, and at the end of die semester, you will be allowed to take home the remaining plants in your herb garden.
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