Other Activities

1. For Exercise A, your instructor may also have onion

root dps available for preparing chromosome squashes. Follow the same procedure as for the VUta faba roots.

2. For Exercise C on cloning herbs, try rooting some of the cuttings in water. Some plants readily root in water, while others do not. Plants rooted in water should be transferred to a porting mixture as soon as the roots are visible.

NAME DATE

LAB SECTION NUMBER

WORKSHEET 2-1 EXERCISE B: CLONES FROM TISSUE CULTURE

Medium

Date

Description of Tissue Culture

Callus culture medium

Expiants placed in culture

Callus culture medium

Callus culture medium

Callus culture medium

Differentiation medium

Differentiation medium

Differentiation medium

Differentiation medium

Differentiation medium

Differentiation medium

Differentiation medium

  1. When did callus growth begin?
  2. When did roots first appear?
  3. When did leaves first appear?
  4. How many plandets developed per culture?

NAME DATE

LAB SECTION NUMBER

WORKSHEET 2-2 EXERCISE C: CLONING HERBS FROM CUTTINGS

TA B L E I INDICATE WHETHER ROOTS WERE PRESENT OR NOT FOR THE HERBS YOU USED.

Name of Herb Used

Treated with Rootone

Untreated Control

MB

TABLE 2 PERCENT OF HERB CUTTINGS DEVaOP'NG ROOTS: SUMMARY OF CLASS RESULTS

Percent of Cuttings with Roots

TABLE 2 PERCENT OF HERB CUTTINGS DEVaOP'NG ROOTS: SUMMARY OF CLASS RESULTS

Percent of Cuttings with Roots

Name of Herb

Treated with Rootone

Untreated Controls

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