Exercise B The Nitrogen Goes Round And Round
- To prepare crystal violet stock solution, weigh out 13.87 g of crystal violet dye. Add 100 ml of 95% cthanol. Stir. Filter through filter paper. Dilute 10 ml of the stock solution with 90 ml of water.
- Dig up field clover or grow beans (Phaseolus vulgaris) and inoculate the soil with Rhizobium powder. Rhizobium powders (AA-15-7832) arc available from Carolina Biological or from local nurserv ccntcrs. •
- Alternatively, prepared microscope slides of RJjizo-bium in legume nodules can be purchased from Carolina Biological or Ward's.
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