Exercise B The Nitrogen Goes Round And Round

  1. To prepare crystal violet stock solution, weigh out 13.87 g of crystal violet dye. Add 100 ml of 95% cthanol. Stir. Filter through filter paper. Dilute 10 ml of the stock solution with 90 ml of water.
  2. Dig up field clover or grow beans (Phaseolus vulgaris) and inoculate the soil with Rhizobium powder. Rhizobium powders (AA-15-7832) arc available from Carolina Biological or from local nurserv ccntcrs. •
  3. Alternatively, prepared microscope slides of RJjizo-bium in legume nodules can be purchased from Carolina Biological or Ward's.

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